Lighting correction for photomicrographs

  • 1
  • Question
  • Updated 5 years ago
Dear Sir;
One feature I would like to see offered is a correction for photographs taken through a microscope. Pictures have an illumination defect seldom seen in street cameras. At times, particularly low magnification (1x, 2x and 4x) exposure produce an uneven illumination. The is a vignetting-like situation in which the central portion is exposed correctly but the periphery has a darker circular shadow. This happens after careful Kohler adjustment.
I appreciate if there is a tool to deal with this problem or it can be developed.
Sincerely,
Daniel J Santa Cruz, MD
Photo of Daniel Santa Cruz

Daniel Santa Cruz

  • 2 Posts
  • 0 Reply Likes

Posted 5 years ago

  • 1
Photo of Chris Cox

Chris Cox

  • 20280 Posts
  • 813 Reply Likes
Which product are you asking for this to be added to?

In general, microscopy and astronomy are too device/situation specific to have general corrections.

But in Photoshop, you can correct for uneven lighting using a white field photo and the divide blend mode. A black field photo can also be subtracted (using the subtract blend mode) from the image to correct for low light noise and amplifier noise in digital sensors.

Unfortunately we don't currently have a good way to correct for radial changes in focus found in many microscope camera adapters.
Photo of Daniel Santa Cruz

Daniel Santa Cruz

  • 2 Posts
  • 0 Reply Likes
Thank you. Very useful